Add in the system Path:
C:\Program Files (x86)\OpenCV-2.1.0\build\bin\Debug
Project->Project Property->Configuration Properties->VC++Directories ->Include Directories:
C:\Program Files (x86)\OpenCV-2.1.0\include\opencv.
Project->Project Property->Configuration Properties->VC++Directories ->Library Directories:
C:\Program Files (x86)\OpenCV-2.1.0\build\lib\Debug
Project->Project Property->Configuration Properties->Linker->Input:
cvaux210d.lib
highgui210d.lib
cv210d.lib
ml210d.lib
cxts210d.lib
cxcore210d.lib
opencv_ffmpeg210d.lib
Configuration of OpenCV2.1.0 with VS2010
原文：http://www.cnblogs.com/grandyang/p/4257718.html