? ? ?bowtie2-build genome.fasta genome
3 ? ? santools依赖库:ZLIB_1.2.3.3,需要更新libz.so.1版本,zlib-1.2.3 版本不够高,需要安装1.2.8版本zlib,安装路径:/data_center_01/soft/zlib-1.2.8/libz.so.1,添加到bashrc的LD_LIBRARY_PATH中
4 ? ? boost c++ library:CPLUS_INCLUDE_PATH=$CPLUS_INCLUDE_PATH:/data_center_01/home/NEOLINE/lihang/Programe/Package/boost_1_55_0/include/
4 ? tophat下载:官网:http://ccb.jhu.edu/software/tophat/index.shtml
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tophat使用参考经典protocol文章:Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks?
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